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KMID : 1101320090410040153
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Korean Journal of Clinical Laboratory Science
2009 Volume.41 No. 4 p.153 ~ p.157
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Real-Time PCR Analysis of SHV Extended-Spectrum beta-Lactamases Producing Klebsiella pneumoniae
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Yang Byoung-Seon
Yook Keun-Dol
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Abstract
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The production of extended-spectrum β-lactamases (ESBLS) of the TEM or SHV type by bacterial pathogens is a major threat to the use of the clinically important expanded-spectrum cephalosporins. The characterization of the SHV ESBLs producing Klebsiella pneumoniae strains present in clinical isolates is time-consuming processes. We describe here in the development of a novel system, which consists of a real time PCR. We found 11 K. pneumoniae strains to be presumptive strains ESBLs producers by clinical and laboratory standards institute (CLSI) guidelines. The double disk synergy test showed 8 ESBL positive and conventional PCR showed 10 SHV ESBL positive, which were K. pneumoniae strains isolates. By real time PCR analysis, SHV gene in 11 of 11 strains were identified. When sequencing analysis was compared with real time PCR, both analysis were presented 99% similarity. In this study, we used a rapid, sensitive, and specific real-time PCR (RT-PCR) method for detection of the assay SHV ESBL producing K. pneumoniae strains in clinical isolates.
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KEYWORD
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Klebsiella pneumoniae, Extended-spectrum β-lactamases, Real-time polymerase chain reaction
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